SEVENTH FRAMEWORK PROGRAMME (FP7)

WELCOME to the P4FIFTY website. 

P4FIFTY is an FP7 funded European Marie Curie Training network of academic and industrial researchers looking to develop enzymatic methods for green oxidation chemistry through the isolation, redesign and application of cytochrome P450 enzymes.
P4fifty
 
 News and Events

 
PRESS RELEASE:
conclusion of the P4FIFTY project
.
 

Justyna Kulig atInternational Science Festival in GothenburgMaria M Razalan and Claudia Spandolf, ESRs at the University of York were two of the organizers of an exhibition at ‘YorNight’ the European Researchers Night which is a mega event which takes place every year simultaneously in several hundred cities all over Europe and beyond. ... read more
   
   
Justyna Kulig atInternational Science Festival in Gothenburg Justyna Kulig, Researcher at AstraZeneca gave a presentation at the International Science Festival in Gothenburg (13-24 04 15) ... read more

 

Krutika Bavishi, University of Copenhagen presenting a lecture at the International  P450 Conference, Kyoto 2014Krutika Bavishi, University of Copenhagen presenting a lecture at the International  P450 Conference, Kyoto 2014 ... read more

 

Professor Birger Lindberg Moller and his ESR Krutika Bavishi at the University of Copenhagen featured in a video made by ASCB TV for their Americal Society for Cell Biology International Conference, 2014 which featured our group, Centre for Synthetic Biology (bioSYNergy)

Martina Picmanova, attended the 62nd Congress and Annual Meeting of Society for Medicinal Plants and Natural Product Research GA2014 in Portuguese GuimarãesMartina Picmanova, attended the 62nd Congress and Annual Meeting of Society for Medicinal Plants and Natural Product Research GA2014 in Portuguese Guimarães ... read more

 

Anja Eichler, ESR at Manchester at the Science Exhibition, Manchester Science Festival (November 2013)Anja Eichler, ESR at Manchester at the Science Exhibition, Manchester Science Festival (November 2013) presenting, The complex life of sugars. ... read more

 

New and Events archive

 
 Publications
  • Bavishi, K. & Hatzakis, N. (2014) Shedding light on protein folding, structural and functional dynamics by single molecule studies. Molecules 19(12):19407-19434.
  • Gricman, L., Vogel, C. & Pleiss, J. (2014) Conservation analysis of class-specific positions in cytochrome P450 monooxygenases: functional and structural relevance. Proteins 82:491-504
  • Miettinen, K., Dong, L., Navrot, N., Schneider, T., Burlat, V., Pollier, J., Woittiez, L., van der Krol, S., Lugan, R., Ilc, T., Verpoorte, R., Oksman-Caldentey, K-M., Martinoia, E., Bouwmeester, H., Goossens, A., Memelink, J. & Werck-Reichhart, D. (2014) The seco-iridoid pathway from Catharanthus roseus. Nat. Commun. 5:3606.
  • Pičmanová, M., Neilson, E.H., Motawia, M.S., Sánchez-Pérez, R., Olsen, C.E., Lindberg Møller, B., Jørgensen, K. & Bjarnholt, N. (2014) Co-occurrence of cyanogenic glucosides and their derivatives as a common feature in metabolic profiles of almond and cassava. Planta Medica 80 (conference abstract P1M17).
  • Website:  www.cyped.biocatnet.de (Version 6.0, December 2014)
  • Bertram, N., Laursen, T., Barker, R., Bavishi, K., Møller, B.L. & Cardenas, M. (2015) Nanodisc films for membrane protein studies by neutron reflection: effect of the protein scaffold choice. Langmuir 31 (30):8386-8391.
  • Gricman, L., Vogel, C. & Pleiss, J. (2015) Identification of universal selectivity-determining positions in cytochrome P450 monooxygenases by systematic literature mining. Proteins 83:1593-603.
  • Kelly, P., Eichler, A., Herter, S., Kranz, D.C., Turner, N.J. & Flitsch, S.L. (2015) Active site diversification of P450cam with indole generates catalysts for benzylic oxidation reactions. Beilstein J. Org. Chem. 11:1713-1720.
  • Kiss, F.M., Khatri, Y., Zapp, J. & Bernhardt, R. (2015) Identification of new substrates for the CYP106A1-mediated 11-oxidation and investigation of the reaction mechanism. FEBS Lett. 589(18):2320-2326.
  • Kiss, F.M., Lundemo, M.T., Zapp, J., Woodley, J.M. & Bernhardt, R. (2015) Process development for the production of 15β-hydroxycyproterone acetate using Bacillus megaterium expressing CYP106A2 as whole-cell biocatalyst. Microb. Cell Fact. 14:28.
  • Kiss, F.M., Schmitz, D., Zapp, J., Dier, T.K., Volmer, D.A. & Bernhardt, R. (2015) Comparison of CYP106A1 and CYP106A2 from Bacillus megaterium – identification of a novel 11-oxidase activity. Appl. Microbiol. Biotechnol. 99(20):8495-8514.
  • Kulig, J.K., Spandolf, C., Hyde, R., Ruzzini, A.C., Eltis, L.D., Grönberg, G., Hayes, M.A. & Grogan, G. (2015) A P450 fusion library of heme domains from Rhodococcus jostii RHA1 and its evaluation for the biotransformation of drug molecules. Bioorg. Med. Chem. 23:5603-5609.
  • Lai, D., Pičmanová, M., Hachem, M.A., Motawia, M.S., Olsen, C.E., Møller, B.L., Rook, F. & Takos, A. (2015) Lotus japonicas flowers are defended by a cyanogenic β-glucosidase with highly restricted expression to essential reproductive organs. Plant Mol. Biol. 89:21-34.
  • Lundemo, M.T., Notonier, S., Streidner, G., Hauer, B. & Woodley, J.M. (2015) Process limitations of a whole-cell P450 catalyzed reaction using a CYP153A-CPR fusion construct expressed in Escherichia coli. Appl. Microbiol. Biotechnol. Oct. epub ahead of print.
  • Lundemo, M.T. & Woodley, J.M. (2015) Guidelines for development and implementation of biocatalytic P450 processes. Appl. Microbiol. Biotechnol. 99:2465-2483.
  • Pičmanová, M., Neilson, E.H., Motawia, M.S., Olsen, C.E., Agerbirk, N., Gray, C.J., Flitsch, S., Meier, S., Silvestro, D., Jørgensen, K., Sánchez-Pérez, R., , Møller, B.L., & Bjarnholt, N. (2015) A recycling pathway for cyanogenic glycosides evidenced by the comparative metabolic profiling in three cyanogenic plant species. Biochem. J. 469:375-389.
  • Van Beek, H.L., Beyer, N., Janssen, D.B. & Fraaije, M.W. (2015) Lyophilization conditions for the storage of monooxygenases. J. Biotechnol. 203:41-44.